serpine2  (R&D Systems)

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 expression is significantly upregulated in COAD. (A) Box plot of SERPINE family genes mRNA expression.in COAD(p < 0.05). (B) Box plot of SERPINE1 and SERPINE2 protein expression in COAD(p < 0.05). (C) Box plot of SERPINE2 mRNA expression among groups of different tumor grades in COAD (p < 0.05).

Article Snippet: Besides, in order to explore the effect of SERPINE2 on macrophage polarization, M0 macrophages were treated with recombinant SERPINE2 (0.4 ng/mL, TargetMol) during 24 hours and then harvested for subsequent experiment.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 expression correlates with tumor immunoregulation, division and proliferation. (A) Volcano map of SERPINE2 co-expressed genes in COAD. (B, C) GO and KEGG enrichment analysis of SERPINE2 co-expressed genes obtained from the cBioPortal, the terms p and q < 0.05 were considered to be significantly enriched. Only 20 leading gene sets are displayed in the plot.

Article Snippet: Besides, in order to explore the effect of SERPINE2 on macrophage polarization, M0 macrophages were treated with recombinant SERPINE2 (0.4 ng/mL, TargetMol) during 24 hours and then harvested for subsequent experiment.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 is positively correlated with M2 macrophage infiltration in COAD. (A) Scatter plot showing the correlation of 6 different immune cell types and SERPINE2 expression (p < 0.05). The black line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (B) Scatter plot showing the correlation of CD68+ Marcophage and SERPINE2 expression (p < 0.05). C.Scatter plot showing the correlation of M1/M2 macrophage and SERPINE2 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (p < 0.05).

Article Snippet: Besides, in order to explore the effect of SERPINE2 on macrophage polarization, M0 macrophages were treated with recombinant SERPINE2 (0.4 ng/mL, TargetMol) during 24 hours and then harvested for subsequent experiment.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 is associated with infiltration of CD68 macrophage in COAD patients. (A) The expression of SEPRINE2 in patient tissues was detected by IHC. Adjacent tissue not expressing SERPINE2; tumor tissue strongly expressing SERPINE2. (B) Scatterplot showing the correlation between SERPINE2 and CD68 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the relationship between SERPINE2 and CD68 expression. (C) The expression of SERPINE2 and CD68 in patient tissues were detected by IHC. High levels of SERPINE2 expression are associated with increased expression of CD68. (* p<0.05, **p<0.01, *** p<0.001).

Article Snippet: Besides, in order to explore the effect of SERPINE2 on macrophage polarization, M0 macrophages were treated with recombinant SERPINE2 (0.4 ng/mL, TargetMol) during 24 hours and then harvested for subsequent experiment.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: The external secretion of SERPINE2 from colorectal cancer cells promotes macrophage polarization to accelerate the development of colorectal cancer. (A) Schema diagram showing that THP-1 cells were treated with PMA, followed by the treatment of SERPINE2 recombinant protein as indicated treatments to obtain activated macrophages. Then tumor cells cocultured with these macrophages. were used to subsequent experiments. (B, C) RT-qPCR and Westen blot displayed SERPINE2 mRNA and Protein expression were upregulated in colorectal cancer cells. (D) The secretion of SERPINE2 in colorectal cancer cells was detected by ELISA. (E) The effect of SERPINE2 recombinant protein on macrophage polarization. (F) RT-qPCR displayed conditioned medium (CM) derived from colorectal cancer cells promotes the upregulation of macrophage M2 markers, compared to normal Intestinal cells. (G) ELISA analysis demonstrated that SERPINE2 secretion was significantly reduced after SERPINE2 knockdown. (H) RT-qPCR displayed conditioned medium with SERPINE2 knockdown down-regulated macrophage M2 markers. (I, J) The conditioned medium from macrophages treated with SERPINE2 recombinant protein promoted cancer cell proliferation (measured by CCK-8 assay) and migration (assessed by Transwell assay. (*p<0.05, **p<0.01, ***p<0.001).

Article Snippet: Besides, in order to explore the effect of SERPINE2 on macrophage polarization, M0 macrophages were treated with recombinant SERPINE2 (0.4 ng/mL, TargetMol) during 24 hours and then harvested for subsequent experiment.

Techniques: Recombinant, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Knockdown, CCK-8 Assay, Migration, Transwell Assay

Journal: Cell proliferation

Article Title: Mechanotransduction Mediated by PDLIM5: The Critical Role of Serpin E2/Integrin β3-Cytoskeleton-Nucleoskeleton Axis in Mechanical Osteogenic Programming.

doi: 10.1111/cpr.70067

Figure Lengend Snippet: FIGURE 8 | The schematic diagram of the regulatory mechanism illustrates that PDLIM5 mediates mechanical stimulation, thereby promoting osteogenic differentiation of human adipose-derived stem cells (hASCs) via the serpin E2/integrin β3-PDLIM5-lamin A signalling axis.

Article Snippet: To knock down PDLIM5 and overexpress PDLIM5 and serpin E2 expression, lentiviruses for PDLIM5- knockdown and PDLIM5overexpression and serpin E2- overexpression and all types of lentiviruses with their corresponding controls were generated by GeneChem (GeneChem Co. Ltd., Shanghai, China).

Techniques: Derivative Assay

Journal: Diabetes Therapy

Article Title: Elevated Serum SERPINE2 Levels are Linked to Impaired Renal Function in Patients with Type 2 Diabetes Mellitus

doi: 10.1007/s13300-025-01742-7

Figure Lengend Snippet: Serum serpin peptidase inhibitor clade E member 2 (SERPINE2) levels were compared between control subjects and patients with type 2 diabetes mellitus (T2DM). a Serum SERPINE2 levels in control subjects and patients with T2DM were detected by enzyme-linked immunosorbent assay (ELISA). The serum SERPINE2 concentration in patients with T2DM ( n = 292) was significantly higher than that in control subjects ( n = 120). Student t test was applied. b Comparison of serum SERPINE2 levels in patients with T2DM with normoalbuminuria, microalbuminuria, and macroalbuminuria. ANOVA was applied. *** P < 0.001

Article Snippet: Serum biomarker levels in control subjects and patients with T2DM were measured using ELISA kits for NGAL (QK1757, R&D Systems), KIM-1 (DSKM100, R&D Systems), TGFβ1 (DY240, R&D Systems), CTGF (DY9190-05, R&D Systems), and antibodies against SERPINE2 (AF2980, R&D Systems) [ ].

Techniques: Control, Enzyme-linked Immunosorbent Assay, Concentration Assay, Comparison

Journal: Diabetes Therapy

Article Title: Elevated Serum SERPINE2 Levels are Linked to Impaired Renal Function in Patients with Type 2 Diabetes Mellitus

doi: 10.1007/s13300-025-01742-7

Figure Lengend Snippet: Correlation between serum serpin peptidase inhibitor clade E member 2 (SERPINE2) and clinical indicators. Pearson correlation test was performed between SERPINE2 with a estimated glomerular filtration rate (eGFR), b serum creatinine (Scr), c neutrophil gelatinase-associated lipocalin (NGAL), d kidney injury molecule 1 (KIM-1), e transforming growth factor-β1 (TGFβ1), and f connective tissue growth factor (CTGF) in all patients with type 2 diabetes mellitus (T2DM)

Article Snippet: Serum biomarker levels in control subjects and patients with T2DM were measured using ELISA kits for NGAL (QK1757, R&D Systems), KIM-1 (DSKM100, R&D Systems), TGFβ1 (DY240, R&D Systems), CTGF (DY9190-05, R&D Systems), and antibodies against SERPINE2 (AF2980, R&D Systems) [ ].

Techniques: Filtration

Journal: Diabetes Therapy

Article Title: Elevated Serum SERPINE2 Levels are Linked to Impaired Renal Function in Patients with Type 2 Diabetes Mellitus

doi: 10.1007/s13300-025-01742-7

Figure Lengend Snippet: Receiver operating characteristic (ROC) curve was used to obtain the optimal cutoff value of serum serpin peptidase inhibitor clade E member 2 (SERPINE2) (278.94 pg/mL) that distinguishes the patients with type 2 diabetes mellitus (T2DM) with and without albuminuria. AUC area under the curve

Article Snippet: Serum biomarker levels in control subjects and patients with T2DM were measured using ELISA kits for NGAL (QK1757, R&D Systems), KIM-1 (DSKM100, R&D Systems), TGFβ1 (DY240, R&D Systems), CTGF (DY9190-05, R&D Systems), and antibodies against SERPINE2 (AF2980, R&D Systems) [ ].

Techniques:

Journal: Diabetes Therapy

Article Title: Elevated Serum SERPINE2 Levels are Linked to Impaired Renal Function in Patients with Type 2 Diabetes Mellitus

doi: 10.1007/s13300-025-01742-7

Figure Lengend Snippet: Schematic diagram of serpin peptidase inhibitor clade E member 2 (SERPINE2) in the development of diabetic nephropathy. Increased SERPINE2 in patients with type 2 diabetes mellitus (T2DM) with renal dysfunction promotes podocyte injury and albuminuria. SERPINE2 also enhances renal fibrosis by promoting fibrosis-related cytokines, such as transforming growth factor-β1 (TGFβ1) and connective tissue growth factor (CTGF). Therefore, the increase in SERPINE2 levels plays an aggravating role in injury of glomerular and tubular cells caused by hyperglycemia and advanced glycation end products (AGEs)

Article Snippet: Serum biomarker levels in control subjects and patients with T2DM were measured using ELISA kits for NGAL (QK1757, R&D Systems), KIM-1 (DSKM100, R&D Systems), TGFβ1 (DY240, R&D Systems), CTGF (DY9190-05, R&D Systems), and antibodies against SERPINE2 (AF2980, R&D Systems) [ ].

Techniques: